Description:
O-glycosylation, a common post-translational modification of serine and threonine residues, regulates biological processes through the direct interactions of glycans with binding proteins. O-glycosylation modulates protein stability, conformation, solubility, and lifespan; however, it has also been implicated in the pathogenesis of a number of diseases, which has stimulated interest in analytical methods capable of isolating O-glycans for further characterization.
The technology is a method termed Rapid Ammonium hydroxide Isobutyric acid O-glycan Deglycosylation Reaction (RAIDR) for the microscale extraction of O-glycans for glycomic characterization. When carried out with the assistance of microwave radiation, efficient O-glycan release can be achieved in 15 minutes; this is the most rapid method of releasing O-linked oligosaccharide reported to date. The RAIDR method also allows for extreme selectivity, resulting in almost exclusive liberation of O-glycans with minimal N-glycans released. Tests have indicated that less than 1% of total N-glycans were liberated during RAIDR release, demonstrating the method's high selectivity for O-glycans. Experiments involving the analysis of human blood serum support the conclusion that the RAIDR protocol represents a rapid, inexpensive, and selective method for the microextraction of O-glycans.
Reference Number: D-1341
Market Applications:
- Pharmaceutical science
- Glycomics research
Features, Benefits, & Advantages:
- Fastest method known for O-linked oligosaccharides release from glycoconjugates
- Inexpensive reagents
- Selective for O-glycans
Intellectual Property: A US. provisional patent, serial number 62423567, was filed on 11/17/16.
Development Stage: The invention has been produced and tested.
Researchers:
- Yehia Mechref, Chemistry and Biochemistry, Texas Tech University, Lubbock, Texas
- Ahmed Abdelmonem, Chemistry and Biochemistry, Texas Tech University, Lubbock, Texas
- Lucas Veillon, Chemistry and Biochemistry, Texas Tech University, Lubbock, Texas
- Byeong Cho, Chemistry and Biochemistry, Texas Tech University, Lubbock, Texas
Keywords: deglycosylation, glycoprotein, O-glycosylation, glycan, glycoconjugate