Gossypium
Hirsutum Tissue-Specific Promoters and their Use
D-0281,
Dr. Randy Allen
Genetic
modification of cotton for agronomic traits requires the use of promoters that
cause the expression of attached genes in specific plant tissues or at certain
developmental times. This patented
technology relates to an isolated promoter-effective DNA molecule of Gossypium
which is operable in embryonic seed tissues and an isolated promoter-effective
DNA molecule of Gossypium which is operable in chlorophyllous tissues. Use of the promoter-effective DNA
molecules and methods of expressing a heterologous mRNA molecule or protein or
polypepetide in chlorophyllous tissue of plants and embryonic seed tissues are
also disclosed.
Competitive
Advantages
Efficient
genetic modification of cotton requires the use of regulatory sequences, known
as promoters that cause the expression of attached genes in specific plant
tissues or at certain developmental times. The pattern of expression must be
analyzed before they can be used to generate commercial transgenic cotton
plants.
Upstream
regulatory sequences of two cotton genes were fused with a GUS reporter gene in
different tissues and developmental stages. Expression of one promoter was detected
only in maturing seeds while expression by the other promoter was seen
predominately in leaves. These
results indicate that these two tissue-specific–promoters can be used to target
the expression of important genes in transgenic cotton plants.
Background
Information/ Patents and/ or Publications
U.S.
Patent has been issued as 7,577,264 on 07/07/2009
Ping
Song, Jeanie Heinen, Teresa Burns, and Randy Allen (2000). Expression of Two Tissue-Specific
Promoters in Transgenic Cotton Plants, Journal of Cotton Science 4:217-223.